Detection of Cholera Toxin-Producing Vibrio cholerae in Phytoplankton from Santubong and Samariang Estuaries
نویسندگان
چکیده
منابع مشابه
Lessons from cholera & Vibrio cholerae
Cholera is an acute form of diarrhoeal disease that plagued human civilization over the centuries. The sudden and explosive onset of the disease in the form of an outbreak or epidemic, coupled with high mortality and morbidity rates, had a tragic impact on the personal as well as social life of people living in the affected areas. The enormity of human sufferings led clinicians and scientists t...
متن کاملRapid screening method for identification of cholera toxin-producing Vibrio cholerae O1 and O139.
A novel method of identifying cholera enterotoxin (CT)-producing Vibrio cholerae serogroups O1 and O139 was developed. The method uses degradation of NAD as a specific biochemical marker for the CT-producing strains. The substrate NAD at a concentration of 100 mumol/liter was markedly degraded when it was incubated at 37 degrees C for 2 h with the CT-producing stains at a final cell density equ...
متن کاملConstruction and characterization of recombinant Vibrio cholerae strains producing inactive cholera toxin analogs.
The catalytic A subunit of cholera toxin (CT-A) is capable of ADP-ribosylating the guanine nucleotide-binding protein, which regulates cell adenylyl cyclase, leading to the life-threatening diarrhea of cholera. Amino acids involved in the enzymatic activity of CT-A have previously been identified. By means of site-directed mutagenesis, an analog of the CT-A subunit gene was created with codon s...
متن کاملMolecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR
Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplific...
متن کاملStructural inferences for Cholera toxin mutations in Vibrio cholerae
Cholera is a global disease that has persisted for millennia. The cholera toxin (CT) from Vibrio cholerae is responsible for the clinical symptoms of cholera. This toxin is a hetero-hexamer (AB(5)) complex consisting of a subunit A (CTA) with a pentamer (B(5)) of subunit B (CTB). The importance of the AB(5) complex for pathogenesis is established for the wild type O1 serogroup using known struc...
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ژورنال
عنوان ژورنال: Borneo Journal of Resource Science and Technology
سال: 2019
ISSN: 0128-2972,2229-9769
DOI: 10.33736/bjrst.1584.2019